A convenient route to mono-trans polyunsaturated free fatty acids

Trans unsaturated fatty acids in humans may be originated both from dietary supplementation and an endogenous free-radical-catalyzed cis−trans isomerization of acid residues naturally occurring cis lipids. The latter process affords geometrical isomers the polyunsaturated mono-trans were demonstrated to connected with stress conditions living organisms. Synthesis is useful for analytical biological research, this case, availability free needed as well possibility mg scale synthetic protocol. Herein, we report a simple route arachidonic acid, eicosapentaenoic docosahexaenoic which includes thiyl radical-catalyzed reaction methyl esters fraction isolation mixture followed by optimization hydrolysis condition purification each acid. Our approach can reach scale, thus fostering more applications biochemical studies.